Overexpression of PeRHD3 alters the root architecture in Populus.
Identifieur interne : 002975 ( Main/Exploration ); précédent : 002974; suivant : 002976Overexpression of PeRHD3 alters the root architecture in Populus.
Auteurs : Meng Xu [République populaire de Chine] ; Wenfan Xie ; Minren HuangSource :
- Biochemical and biophysical research communications [ 1090-2104 ] ; 2012.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Agrobacterium tumefaciens (MeSH), Caulimovirus (génétique), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Phylogenèse (MeSH), Populus (anatomie et histologie), Populus (génétique), Protéines G (biosynthèse), Protéines G (classification), Protéines G (génétique), Racines de plante (anatomie et histologie), Racines de plante (génétique), Racines de plante (ultrastructure), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Séquence conservée (MeSH), Séquence d'acides aminés (MeSH), Transformation génétique (MeSH), Végétaux génétiquement modifiés (anatomie et histologie), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (ultrastructure).
- MESH :
- anatomie et histologie : Populus, Racines de plante, Végétaux génétiquement modifiés.
- biosynthèse : Protéines G.
- classification : Protéines G.
- génétique : ADN complémentaire, Caulimovirus, Populus, Protéines G, Racines de plante, Végétaux génétiquement modifiés.
- ultrastructure : Agrobacterium tumefaciens, Clonage moléculaire, Données de séquences moléculaires, Phylogenèse, Racines de plante, Régions promotrices (génétique), Régulation de l'expression des gènes végétaux, Séquence conservée, Séquence d'acides aminés, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (MeSH), Amino Acid Sequence (MeSH), Caulimovirus (genetics), Cloning, Molecular (MeSH), Conserved Sequence (MeSH), DNA, Complementary (genetics), GTP-Binding Proteins (biosynthesis), GTP-Binding Proteins (classification), GTP-Binding Proteins (genetics), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Roots (anatomy & histology), Plant Roots (genetics), Plant Roots (ultrastructure), Plants, Genetically Modified (anatomy & histology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (ultrastructure), Populus (anatomy & histology), Populus (genetics), Promoter Regions, Genetic (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , biosynthesis : GTP-Binding Proteins.
- chemical , classification : GTP-Binding Proteins.
- chemical , genetics : DNA, Complementary, GTP-Binding Proteins.
- anatomy & histology : Plant Roots, Plants, Genetically Modified, Populus.
- genetics : Caulimovirus, Plant Roots, Plants, Genetically Modified, Populus.
- ultrastructure : Plant Roots, Plants, Genetically Modified.
- Agrobacterium tumefaciens, Amino Acid Sequence, Cloning, Molecular, Conserved Sequence, Gene Expression Regulation, Plant, Molecular Sequence Data, Phylogeny, Promoter Regions, Genetic, Transformation, Genetic.
Abstract
Adventitious rooting is essential for the vegetative propagation of economically important woody species. A better understanding of the genetic and physiological mechanisms that promote or hinder rooting will enhance the potential for successful commercial deployment of trees. ROOT HAIR DEFECTIVE 3 (RHD3), a large GTP-binding protein, is ubiquitously expressed in plants. Our previous microarray study identified differential expression patterns of genes belonging to the RHD3 family during adventitious root development from hardwood cuttings, and indicated that the RHD3 genes were involved in adventitious rooting in Populus. In this study, we cloned and characterized cDNAs of the two Populus RHD3 genes, designated as PeRHD3a and PeRHD3b. Transcripts encoded by the two genes were detected in roots, stems, leaves and petioles. To characterize the cellular functions of the genes, Agrobacterium tumifaciens was used to transform poplar with a vector that places expression of the target gene under the control of the strong constitutive promoter, Cauliflower Mosaic Virus 35S (Pro35S) promoter. Both PeRHD3a transgenic lines and PeRHD3b transgenic lines showed very similar phenotypic characteristics. Overexpression of PeRHD3a or PeRHD3b in poplar plants resulted in the formation of only a single prominent adventitious root with well-developed lateral roots, characteristic abnormalities in the root tip, and longer and more plentiful root hairs. These results imply that RHD3 may control adventitious and lateral root formation, as well as root hair development by regulating anisotropic cell expansion.
DOI: 10.1016/j.bbrc.2012.06.083
PubMed: 22732403
Affiliations:
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Huang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22732403</idno><idno type="pmid">22732403</idno><idno type="doi">10.1016/j.bbrc.2012.06.083</idno><idno type="wicri:Area/Main/Corpus">002983</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002983</idno><idno type="wicri:Area/Main/Curation">002983</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002983</idno><idno type="wicri:Area/Main/Exploration">002983</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Overexpression of PeRHD3 alters the root architecture in Populus.</title><author><name sortKey="Xu, Meng" sort="Xu, Meng" uniqKey="Xu M" first="Meng" last="Xu">Meng Xu</name><affiliation wicri:level="1"><nlm:affiliation>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China. xum@njfu.edu.cn</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation></author><author><name sortKey="Xie, Wenfan" sort="Xie, Wenfan" uniqKey="Xie W" first="Wenfan" last="Xie">Wenfan Xie</name></author><author><name sortKey="Huang, Minren" sort="Huang, Minren" uniqKey="Huang M" first="Minren" last="Huang">Minren Huang</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="eISSN">1090-2104</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (MeSH)</term><term>Amino Acid Sequence 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(génétique)</term><term>Agrobacterium tumefaciens (MeSH)</term><term>Caulimovirus (génétique)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Populus (anatomie et histologie)</term><term>Populus (génétique)</term><term>Protéines G (biosynthèse)</term><term>Protéines G (classification)</term><term>Protéines G (génétique)</term><term>Racines de plante (anatomie et histologie)</term><term>Racines de plante (génétique)</term><term>Racines de plante (ultrastructure)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquence conservée (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (anatomie et histologie)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>GTP-Binding Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>GTP-Binding Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>GTP-Binding Proteins</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Populus</term><term>Racines de plante</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Plant Roots</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines G</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Protéines G</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Caulimovirus</term><term>Plant Roots</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Caulimovirus</term><term>Populus</term><term>Protéines G</term><term>Racines de plante</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Plant Roots</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Conserved Sequence</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Promoter Regions, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Phylogenèse</term><term>Racines de plante</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term><term>Séquence conservée</term><term>Séquence d'acides aminés</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Adventitious rooting is essential for the vegetative propagation of economically important woody species. A better understanding of the genetic and physiological mechanisms that promote or hinder rooting will enhance the potential for successful commercial deployment of trees. ROOT HAIR DEFECTIVE 3 (RHD3), a large GTP-binding protein, is ubiquitously expressed in plants. Our previous microarray study identified differential expression patterns of genes belonging to the RHD3 family during adventitious root development from hardwood cuttings, and indicated that the RHD3 genes were involved in adventitious rooting in Populus. In this study, we cloned and characterized cDNAs of the two Populus RHD3 genes, designated as PeRHD3a and PeRHD3b. Transcripts encoded by the two genes were detected in roots, stems, leaves and petioles. To characterize the cellular functions of the genes, Agrobacterium tumifaciens was used to transform poplar with a vector that places expression of the target gene under the control of the strong constitutive promoter, Cauliflower Mosaic Virus 35S (Pro35S) promoter. Both PeRHD3a transgenic lines and PeRHD3b transgenic lines showed very similar phenotypic characteristics. Overexpression of PeRHD3a or PeRHD3b in poplar plants resulted in the formation of only a single prominent adventitious root with well-developed lateral roots, characteristic abnormalities in the root tip, and longer and more plentiful root hairs. These results imply that RHD3 may control adventitious and lateral root formation, as well as root hair development by regulating anisotropic cell expansion.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22732403</PMID><DateCompleted><Year>2012</Year><Month>11</Month><Day>08</Day></DateCompleted><DateRevised><Year>2012</Year><Month>07</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2104</ISSN><JournalIssue CitedMedium="Internet"><Volume>424</Volume><Issue>2</Issue><PubDate><Year>2012</Year><Month>Jul</Month><Day>27</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation></Journal><ArticleTitle>Overexpression of PeRHD3 alters the root architecture in Populus.</ArticleTitle><Pagination><MedlinePgn>239-44</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bbrc.2012.06.083</ELocationID><Abstract><AbstractText>Adventitious rooting is essential for the vegetative propagation of economically important woody species. A better understanding of the genetic and physiological mechanisms that promote or hinder rooting will enhance the potential for successful commercial deployment of trees. ROOT HAIR DEFECTIVE 3 (RHD3), a large GTP-binding protein, is ubiquitously expressed in plants. Our previous microarray study identified differential expression patterns of genes belonging to the RHD3 family during adventitious root development from hardwood cuttings, and indicated that the RHD3 genes were involved in adventitious rooting in Populus. In this study, we cloned and characterized cDNAs of the two Populus RHD3 genes, designated as PeRHD3a and PeRHD3b. Transcripts encoded by the two genes were detected in roots, stems, leaves and petioles. To characterize the cellular functions of the genes, Agrobacterium tumifaciens was used to transform poplar with a vector that places expression of the target gene under the control of the strong constitutive promoter, Cauliflower Mosaic Virus 35S (Pro35S) promoter. Both PeRHD3a transgenic lines and PeRHD3b transgenic lines showed very similar phenotypic characteristics. Overexpression of PeRHD3a or PeRHD3b in poplar plants resulted in the formation of only a single prominent adventitious root with well-developed lateral roots, characteristic abnormalities in the root tip, and longer and more plentiful root hairs. These results imply that RHD3 may control adventitious and lateral root formation, as well as root hair development by regulating anisotropic cell expansion.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Meng</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China. xum@njfu.edu.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Wenfan</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Minren</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>06</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.6.1.-</RegistryNumber><NameOfSubstance UI="D019204">GTP-Binding Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017796" MajorTopicYN="N">Caulimovirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019204" MajorTopicYN="N">GTP-Binding Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="Y">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="Y">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>05</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>6</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>6</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22732403</ArticleId><ArticleId IdType="pii">S0006-291X(12)01178-3</ArticleId><ArticleId IdType="doi">10.1016/j.bbrc.2012.06.083</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Huang, Minren" sort="Huang, Minren" uniqKey="Huang M" first="Minren" last="Huang">Minren Huang</name><name sortKey="Xie, Wenfan" sort="Xie, Wenfan" uniqKey="Xie W" first="Wenfan" last="Xie">Wenfan Xie</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Meng" sort="Xu, Meng" uniqKey="Xu M" first="Meng" last="Xu">Meng Xu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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